| Primer | Species | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 511 | Brevibacillus brevis | TCAATGCTGCTCGACGGA | GATCGCCTTGCTGAATGCG | 59.03 | 60.01 | 245 | 94 |
100.00%
|
100.00%
|
| Primer 512 | Brevibacillus brevis | AGTCGCCAAACAGCTGCA | AACCTGACCGTTTCCGTCG | 60.20 | 60.30 | 286 | 94 |
100.00%
|
100.00%
|
| Primer 513 | Brevibacillus brevis | GCCTGCTTTTGGCTGATCG | TTCGTCCGAAAGCCGTGT | 59.86 | 59.58 | 249 | 94 |
100.00%
|
100.00%
|
| Primer 514 | Brevibacillus brevis | TGGTATGCACGGGAACTCG | TCAGCTGCGTCAAGTGCT | 59.78 | 59.58 | 187 | 94 |
100.00%
|
100.00%
|
| Primer 515 | Brevibacillus brevis | AATGGTGGACGTAGCTGGC | AACGTTGTCGCCACCCAT | 60.08 | 59.89 | 190 | 93 |
100.00%
|
100.00%
|
| Primer 516 | Brevibacillus brevis | TTCCTGTCGCTGCCATCTG | ACCGCTTCCATTCGCAGT | 60.08 | 59.65 | 188 | 93 |
100.00%
|
100.00%
|
| Primer 517 | Brevibacillus brevis | TCAAACGCATGCGCACAT | GCACCTGTGGAGCTGAAGT | 59.05 | 59.93 | 234 | 93 |
100.00%
|
100.00%
|
| Primer 518 | Brevibacillus brevis | ACTGCACCGCAAGCTCAT | TTCCCAAGCGTCGCAAGT | 59.97 | 59.89 | 212 | 93 |
100.00%
|
100.00%
|
| Primer 519 | Brevibacillus brevis | TGTACACCGTCAAGGCGAC | TGCACCGCTGGCATTGTA | 60.01 | 59.97 | 162 | 93 |
100.00%
|
100.00%
|
| Primer 520 | Brevibacillus brevis | GTACACCGTCAAGGCGACT | TGCACCGCTGGCATTGTA | 59.71 | 59.97 | 161 | 93 |
100.00%
|
100.00%
|